A Family Divided: Distinct Structural and Mechanistic Features of the SpoU-TrmD (SPOUT) Methyltransferase Superfamily
نویسندگان
چکیده
منابع مشابه
Mechanistic features of the atypical tRNA m1G9 SPOUT methyltransferase, Trm10
The tRNA m1G9 methyltransferase (Trm10) is a member of the SpoU-TrmD (SPOUT) superfamily of methyltransferases, and Trm10 homologs are widely conserved throughout Eukarya and Archaea. Despite possessing the trefoil knot characteristic of SPOUT enzymes, Trm10 does not share the same quaternary structure or key sequences with other members of the SPOUT family, suggesting a novel mechanism of cata...
متن کاملTransfer RNA methyltransferases with a SpoU-TrmD (SPOUT) fold and their modified nucleosides in tRNA
The existence of SpoU-TrmD (SPOUT) RNA methyltransferase superfamily was first predicted by bioinformatics. SpoU is the previous name of TrmH, which catalyzes the 2'-Omethylation of ribose of G18 in tRNA; TrmD catalyzes the formation of N1-methylguanosine at position 37 in tRNA. Although SpoU (TrmH) and TrmD were originally considered to be unrelated, the bioinformatics study suggested that the...
متن کاملtRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily
TrmJ proteins from the SPOUT methyltransferase superfamily are tRNA Xm32 modification enzymes that occur in bacteria and archaea. Unlike archaeal TrmJ, bacterial TrmJ require full-length tRNA molecules as substrates. It remains unknown how bacterial TrmJs recognize substrate tRNAs and specifically catalyze a 2'-O modification at ribose 32. Herein, we demonstrate that all six Escherichia coli (E...
متن کاملSPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases.
Nucleotide modification is a major source of structural and biochemical diversity of RNAs, particularly of rRNAs and tRNAs (Rozenski et al., 1999). Basespecific methylation is one of the prevalent modes of post-transcriptional modification along with pseudouridylation and thio-uridylation. The catalytic domains of the majority of RNA methylases belong to the vast la rge superc lass o f Rossmanf...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2018
ISSN: 0006-2960,1520-4995
DOI: 10.1021/acs.biochem.8b01047